Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Cedrus deodara (Pinaceae) Root Oil on the Histopathology of Rat Liver and Kidney

Rehana Parveen¹, Muhammad A Azmi² , Syed Naeemul H Naqvi¹, Syed M Mahmood³, Ijaz H Zaidi⁴

¹Department of Pharmacology, Institute of Pharmaceutical Sciences; ²Department of Physiology, Baqai Medical University, 51 Deh Tor, Gadap Road, Near Toll Plaza, Super Highway, P.O. Box 2407, Karachi-74600; ³Department of Oncology, Agha Khan University Hospital, National Stadium Road; ⁴Department of Casualty, P.N.S. Shifa Hospital, DHA, Phase 2, Karachi, Pakistan.

For correspondence:- Muhammad Azmi Email: azmiahmed@hotmail.com Tel:+92214410434

Received: 15 October 2009 Accepted: 25 February 2010 Published: 15 April 2010

Citation: Parveen R, Azmi MA, H Naqvi SN, Mahmood SM, Zaidi IH. Effect of Cedrus deodara (Pinaceae) Root Oil on the Histopathology of Rat Liver and Kidney. Trop J Pharm Res 2010; 9(2):127-133 doi: 10.4314/tjpr.v9i2.3

© 2010 The authors.
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Abstract

Purpose: To evaluate the effect of Cedrus deodara (Pinaceae) root oil, used traditionally for the treatment of various ailments, on the histopathology of the liver and kidney of Wistar albino rats.

Methods: The oil was extracted from the plant root by dry destructive distillation method and administered orally at doses of 0.5 ml and 2.5 ml per rat. Each animal group was comprised of 30 rats. Treated and untreated group of rats were sacrificed and organs (liver and kidney) were taken out for tissue processing and staining with haematoxylin and eosin according to the method of Bancroft and Stevens. Microscopic examination was then carried out to observe any pathological changes in the animals.

Results: The root oil produced atrophic changes and haemolysis in the glomerular region as well as shrinkage and shedding of glomerular epithelium in the kidney tissues. Marked dilation of the central vein and congested blood vessels in the rat liver at both doses tested was also seen.

Conclusion: The root oil of C. deodara (Pinaceae), at the doses administered, produced some adverse effects on the tissues tested but these effects were not lethal. Some toxic changes in the tissues of liver and kidney were also found.

Keywords: Histopathology, Cedrus deodara root oil, Liver, Kidney, Albino rats